The right ventricle forms the largest part of the anterior surface of the heart, a small part of the diaphragmatic surface, and almost the entire inferior border of the heart. Superiorly it tapers into an arterial cone, the conus arteriosus (infundibulum), which leads into the pulmonary trunk. The interior of the right ventricle has irregular muscular elevations (trabeculae carneae). A thick muscular ridge, the supraventricular crest, separates the ridged muscular wall of the inflow part of the chamber from the smooth wall of the conus arteriosus, or outflow part. The inflow part of the ventricle receives blood from the right atrium through the right AV (tricuspid) orifice, located posterior to the body of the sternum at the level of the 4^th and 5^th intercostal spaces. The right AV orifice is surrounded by one of the fibrous rings of the fibrous skeleton of the heart. The fibrous ring keeps the caliber of the orifice constant (large enough to admit the tips of three fingers), resisting the dilation that might otherwise result from blood being forced through it at varying pressures.

The tricuspid valve guards the right AV orifice. The bases of the valve cusps are attached to the fibrous ring around the orifice. Because the fibrous ring maintains the caliber of the orifice, the attached valve cusps contact each other in the same way with each heartbeat. Tendinous cords (Latin chordae tendineae) attach to the free edges and ventricular surfaces of the anterior, posterior, and septal cusps, much like the cords attaching to a parachute. The tendinous cords arise from the apices of papillary muscles, which are conical muscular projections with bases attached to the ventricular wall. The papillary muscles begin to contract before contraction of the right ventricle, tightening the tendinous cords and drawing the cusps together. Because the cords are attached to adjacent sides of two cusps, they prevent separation of the cusps and their inversion when tension is applied to the tendinous cords and maintained throughout ventricular contraction (systole) - that is, the cusps of the tricuspid valve are prevented from prolapsing (being driven into the right atrium) as ventricular pressure rises. Thus, regurgitation of blood (backward flow of blood) from the right ventricle back into the right atrium is blocked during ventricular systole by the valve cusps.

Three papillary muscles in the right ventricle correspond to the cusps of the tricuspid valve:

1. The anterior papillary muscle, the largest and most prominent of the three, arises from the anterior wall of the right ventricle; its tendinous cords attach to the anterior and posterior cusps of the tricuspid valve.

2. The posterior papillary muscle, smaller than the anterior muscle, may consist of several parts; it arises from the inferior wall of the right ventricle, and its tendinous cords attach to the posterior and septal cusps of the tricuspid valve.

3. The septal papillary muscle arises from the interventricular septum, and its tendinous cords attach to the anterior and septal cusps of the tricuspid valve.

The interventricular septum (IVS), composed of muscular and membranous parts, is a strong, obliquely placed partition between the right and left ventricles, forming part of the walls of each. Because of the much higher blood pressure in the left ventricle, the muscular part of the IVS, which forms the majority of the septum, has the thickness of the remainder of the wall of the left ventricle (two to three times as thick as the wall of the right ventricle) and bulges into the cavity of the right ventricle. Superiorly and posteriorly, a thin membrane, part of the fibrous skeleton of the heart, forms the much smaller membranous part of the IVS. On the right side, the septal cusp of the tricuspid valve is attached to the middle of this membranous part of the fibrous skeleton. This means that inferior to the cusp, the membrane is an interventricular septum, but superior to the cusp it is an atrioventricular septum, separating the right atrium from the left ventricle.

The septomarginal trabecula (moderator band) is a curved muscular bundle that traverses the right ventricular chamber from the inferior part of the IVS to the base of the anterior papillary muscle. This trabecula is important because it carries part of the right branch of the AV bundle, a part of the conducting system of the heart to the anterior papillary muscle. This "shortcut" across the chamber seems to facilitate conduction time, allowing coordinated contraction of the anterior papillary muscle.

The right atrium contracts when the right ventricle is empty and relaxed; thus blood is forced through this orifice into the right ventricle, pushing the cusps of the tricuspid valve aside like curtains. The inflow of blood into the right ventricle (inflow tract) enters posteriorly; and when the ventricle contracts, the outflow of blood into the pulmonary trunk (outflow tract) leaves superiorly and to the left. Consequently, the blood takes a U-shaped path through the right ventricle, changing direction about 140°. This change in direction is accommodated by the supraventricular crest, which deflects the incoming flow into the main cavity of the ventricle, and the outgoing flow into the conus arteriosus toward the pulmonary orifice. The inflow (AV) orifice and outflow (pulmonary) orifice are approximately 2 cm apart. The pulmonary valve at the apex of the conus arteriosus is at the level of the left 3^rd costal cartilage.